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在野外帐蓬接种室里,预先用Cronartium quercuum f. sp. fusiforme接种无锈病的火炬松幼苗,然后将接种苗栽培起来,并建成两个种植圃。十年以后,在第一个种植圃里感病率是22％,而未接种的对照感病率是29％。在另一个种植圃里,感病率与原来接种的相同家系的锈病感病率有关。在这两个种植圃里,将接种试验中感病率较低的家系的无锈病成活植株用于野外栽培,一般都能保持无病,而易感病家系的许多无病成活植株,在野外栽培后,同样易感病。在子代试验中,人为地对两个种植圃中八株自由援粉的无病成活的幼苗子代接种,结果,从原来接种的抗病家系中,获得了唯一的高度抗病的子代,其余的都是易感病的。
In field tents vaccination room, rust-free petunia seedlings were inoculated beforehand with Cronartium quercuum f. Sp. Fusiforme. The seedlings were then cultivated and planted into two plantations. Ten years later, the prevalence was 22% in the first plantation and 29% in the unvaccinated control. In another nursery, the prevalence was related to the prevalence of the rust in the same pedigree as originally vaccinated. In both plantations, the rust-free surviving plants of the low-susceptible families in the inoculation were used for field cultivation, generally maintaining disease-free, disease-free survival of many disease-free surviving plants of the susceptible pedigree in the wild After cultivation, the same susceptible to disease. In offspring trials, eight offspring of disease free surviving seedlings from two plantations were artificially inoculated, and as a result, the only highly resistant progeny were obtained from the originally inoculated resistant families , The rest are susceptible to disease.