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目的研究DNATyperTM15试剂盒在法医学中的应用。方法共收集60种8137份检材,在34个实验室,用5种不同方法提取DNA,使用DNATyperTM15试剂盒与2种同类进口试剂扩增并对比检测。结果DNATyperTM15试剂盒对不同提取方法、检材种类、扩增与检测仪器、操作环境、操作人员等均具有适应性。结论DNATyperTM15试剂盒能够用于法医学检验鉴定。
Objective To study the application of DNATyperTM15 kit in forensic science. Methods A total of 60 samples of 8137 samples were collected. DNA was extracted by 5 different methods in 34 laboratories. DNA was amplified by DNATyperTM15 kit and two imported reagents of the same type and compared with each other. Results DNATyperTM15 kit for different extraction methods, the type of samples, amplification and detection equipment, operating environment, operators and so on are adaptable. Conclusion DNATyperTM15 kit can be used for forensic test identification.