目的:优化痛风消颗粒的水提醇沉工艺。方法:采用正交试验法,以盐酸小檗碱、丹酚酸B含量为指标,考察加水量、提取时间、提取次数对水提取工艺的影响;以盐酸小檗碱、丹酚酸B保留率为指标,考察提取液相对密度、含醇量、醇沉静置时间对醇沉工艺的影响;同时进行验证试验。结果:最优水提取工艺为加8倍量水、煎煮3次、每次60 min;最优醇沉工艺为提取液浓缩至相对密度1.15、冷却后加入乙醇使含醇量为80%、静置48 h,除去沉淀。验证试验结果表明,提取后小檗碱、丹酚酸B含量平均值分别为6.344 6、7.312 2 mg/g(RSD=2.46%、1.08%,n=3),保留率分别为91.03%、92.39%(RSD=1.72%、1.50%,n=3)。结论:优化的水提、醇沉工艺稳定可行、重现性好,适用于本制剂的提取、分离和纯化。 Objective: To optimize the process of water extraction and alcohol precipitation of gout powder. Methods: By orthogonal test, taking berberine hydrochloride and salvianolic acid B content as indexes, the effects of water addition, extraction time and extraction times on water extraction process were investigated. Taking berberine hydrochloride, salvianolic acid B retention rate As an indicator to examine the relative density of the extract, the alcohol content, the alcohol settling time alcohol precipitation process; at the same time validation test. Results: The optimal water extraction process was adding 8 times the amount of water, boiling 3 times, each time 60 min; the optimal alcohol precipitation process for the extract was concentrated to a relative density of 1.15, after cooling ethanol was added to make the alcohol content of 80% Let stand for 48 h, remove the sediment. The validation test results showed that the average contents of berberine and salvianolic acid B were 6.344 6,7.312 2 mg / g (RSD = 2.46%, 1.08%, n = 3) respectively, the retention rates were 91.03%, 92.39 % (RSD = 1.72%, 1.50%, n = 3). Conclusion: The optimized water extraction and alcohol precipitation process is stable and feasible with good reproducibility and is suitable for the extraction, separation and purification of this preparation.