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为了研究酶解法辅助提取地锦草中槲皮素的工艺,试验采用Box-Behnken试验设计和响应面法研究了纤维素酶用量、酶解温度、酶解p H值、酶解时间对槲皮素得率的影响。结果表明:最佳酶解提取工艺条件为纤维素酶用量0.6%、酶解温度50℃、酶解p H值4.5、酶解时间45 min。在该酶解条件下,槲皮素得率为1.87 mg/g,与理论预测值(1.88 mg/g)的相对误差仅为0.63%。说明通过Box-Behnken试验设计和响应面法得到的优化工艺参数比较准确,具有实用性。
In order to study the technology of enzyme-assisted extraction of quercetin in Euphorbiaceae, the Box-Behnken experimental design and response surface methodology were used to study the effects of cellulase dosage, enzymatic hydrolysis temperature, enzymatic hydrolysis p H value and enzymolysis time on quercetin The impact of prime rate. The results showed that the optimum conditions of enzymatic extraction were as follows: cellulase dosage 0.6%, enzymatic hydrolysis temperature 50 ℃, p H value 4.5 and enzymolysis time 45 min. Under this condition, the yield of quercetin was 1.87 mg / g, which was only 0.63% relative to the theoretical value of 1.88 mg / g. It shows that the optimized process parameters obtained by Box-Behnken experimental design and response surface method are more accurate and practical.