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目的:探讨心气虚证动物模型的建立方法。方法:BALB/c小鼠随机分为模型组20只,药物反证组10只,正常组20只,均为雌雄各半。采用基础进食量16天,采用电动跑台强迫小鼠跑步至力竭,并结合大剂量灌服心得安2.4mg/100g等综合方法建立心气虚证动物模型,补心气药物反证组对所建立模型予以验证。指标观测结果同正常组小鼠进行统计学比较。结果:模型组小鼠心脏收缩、舒张功能减退,细胞免疫功能下降,心肌存在着微小损伤,均与临床心气虚证的改变相似;药物反证组小鼠心脏收缩、舒张功能得到了一定修复。结论:采用基础进食量、电刺激强迫跑步及大剂量灌服心得安的综合方法建立心气虚证动物模型是可行的。
Objective: To explore the establishment of animal models of heart qi deficiency syndrome. Methods: BALB / c mice were randomly divided into model group (n = 20), drug anti-syndrome group (n = 10) and normal group (n = 20). The basic feeding amount was 16 days. The electric running treadmill was used to force the mice to run to exhaustion. Combined with the large dose of 2.4mg / 100g of propranolol, the animal model of heart qi deficiency syndrome was established. Be verified. The index observation results were statistically compared with the normal mice. Results: The contraction and relaxation of cardiac function of mice in model group decreased, the cellular immune function decreased and there was slight damage in myocardium, all of which were similar to the change of clinical syndrome of cardiocardiopathy. The contractile and diastolic function of anti-cardioprotective mice was repaired. Conclusion: It is feasible to establish the animal model of heart qi deficiency syndrome by the comprehensive method of basal intake, electrical stimulation of forced running and high dose of oral gavage.