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目的 连续追踪传染性非典型肺炎 [又称严重急性呼吸综合征 (SARS) ]患者 1年 ,了解其血清SARS病毒特异性IgM、IgG及N蛋白抗体滴度变化 ;追踪检测未发病者的SARS血清特异性抗体。方法 用IFA和双抗原夹心ELISA方法连续检测SARS患者发病后第 7、14、30、6 0、12 0、180、2 10、2 70、36 0天血清特异性抗体滴度。结果 间接免疫荧光染色法 (IFA)检测 14例SARS患者血清特异性IgG抗体在第 7天检出 ,滴度为 1/ 4 0 ,12 0d达高峰平均滴度 1/ 112 0 ,180d开始下降平均滴度 1/ 2 74 ,2 10d平均滴度 1/ 16 3,1年后平均滴度维持在 1/ 71。特异性抗体IgM滴度第 7天均为阴性 ,14d检出平均滴度为 1/ 32 ,30d检出达高峰 ,6 0d滴度开始下降 1/ 86 ,12 0d后大部分患者IgM滴度消失。N蛋白抗体 7d检出平均滴度为 1/ 5 7,12 0d达高峰平均滴度 1/ 319,2 10d后N蛋白抗体平均滴度 1/ 187,2 70d平均滴度 1/ 134,1年后平均滴度 1/ 84 ,高于IgG抗体水平。与患者密切接触的 10名亲属 (包括 2名儿童 ) ,检测血清中SARS特异性抗体 ,IgM、IgG抗体及N蛋白抗体全部为阴性。结论 观察 14例家庭聚集患者血清SARS病毒特异性IgG、IgM抗体 ,1年后仍维持在一定水平 ,可为疫苗的研制提供指导。 10名密切接触者血清中未检出SARS病毒特异性抗体?
OBJECTIVE: To continuously track the SARS patients for one year and to understand the changes of antibody titer of specific IgM, IgG and N of serum SARS virus. To track the SARS serum of uninfected patients Specific antibodies. Methods Serum specific antibody titers of 7th, 14th, 30th, 60th, 120th, 180th, 210th, 70th and 70th day after onset of SARS were detected by IFA and double antigen sandwich ELISA. Results Serum specific IgG antibody was detected on the 7th day in indirect immunofluorescence staining (IFA) in 14 SARS patients. The titer was 1/40 on day 0. The average peak titer was 1/112 0 on day 12 0, and decreased from 180 days Titers 1/2 74, 2 10d average titer 1/16 3, 1 year after the average titers remained at 1/71. The IgM titers of all the specific antibodies were all negative on the 7th day. The average titers were 1/32 on the 14th day, the highest on the 30th day and the 1/6th day on the 60th day. The IgM titers of most patients disappeared after 12 days . The average titer of N protein antibody detected at 7d was 1 / 57,12d and reached the peak average titer of 1 / 319,2 After 10 days, the average titer of N protein antibody was 1 / 187,270d. The average titer was 1/134, 1 year After the average titer 1/84, higher than the level of IgG antibodies. Ten relatives (including two children) who were in close contact with the patient were all negative for SARS-specific, IgM, IgG, and N-protein in serum. Conclusions Serum SARS virus-specific IgG and IgM antibodies were observed in 14 cases of familial aggregation patients and remained at a certain level after 1 year, which may provide guidance for the development of the vaccine. SARS virus-specific antibodies were not detected in the serum of 10 close contacts?