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本文以褐藻酸降解菌为病原体研究褐藻酸降解菌侵染海带的小孢子体不同时期体内的活性氧——超氧负离子自由基 (O-·2 )、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、抗坏血酸过氧化物酶 (APX)和抗坏血酸 (ASA)等的变化。实验结果表明 :海带活性氧 (O-·2 )含量在褐藻酸降解菌感染初期表现为升高 ,随后表现为下降。并且 ,随着褐藻酸降解菌侵染浓度的升高 ,活性氧 (O-·2 )含量呈现上升趋势 ,推测活性氧 (O-·2 )是海带抗褐藻酸降解菌感染的有效防御手段之一。SOD,CAT,ASA在感染的初期起着积极的抵抗褐藻酸降解菌感染的作用 ;APX在褐藻酸降解菌感染的前期没有发挥其抗感染的作用 ,而是在后期显示出了一定的抗感染作用。此结果可为海带病害的机理及幼苗集约化培养过程中病害的防治提供科学的依据。
In this paper, alginate-degrading bacteria were used as pathogens to study the changes of reactive oxygen species (ROS), superoxide anion radical (SOD) and superoxide dismutase (SOD) in microspores of kelp infected alginate- Catalase (CAT), ascorbate peroxidase (APX) and ascorbic acid (ASA) and so on. The experimental results showed that the content of reactive oxygen species (O-2) of kelp increased in the early stage of infection with alginic acid-degrading bacteria, and then decreased. Moreover, as the concentration of alginic acid-degrading bacteria increased, the content of reactive oxygen species (O- · 2) showed an upward trend. It is speculated that active oxygen (O- · 2) is an effective defensive method for kelp resistant to alginate-degrading bacteria infection one. SOD, CAT, ASA played an active role in the infection of alginic acid-degrading bacteria in the early stage of infection. APX did not play an anti-infection role in the early stage of alginic acid-degrading bacteria infection, but showed certain anti-infection later effect. This result can provide a scientific basis for the mechanism of kelp disease and the prevention and treatment of disease in the intensive culture of seedlings.