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目的探讨采用TMS-MEP评价大鼠脊髓损伤后运动功能障碍的可行性,并评价其在法医学中的应用价值。方法 SD雌性大鼠100只,分为3个损伤组、1个假手术组和1个正常对照组。损伤组采用Allen’s打击装置制作脊髓损伤模型;各组均于实验前、损伤后1d、1周、2周、4周,根据大鼠脊髓运动功能评定标准进行运动功能评分,并在70%刺激强度下记录腓肠肌的TMS-MEP;采用SPSS 19.0软件进行数据处理,单因素方差分析比较组间差异。结果损伤组随打击能量的增加,脊髓损伤程度加重,双下肢运动功能缺失明显,与正常对照组、假手术组和实验前之间均存在显著性差异(P<0.05)。损伤组MEP潜伏期和波幅较正常对照组均延长,差异具有显著意义(P<0.05)。TMS-MEP回归方程Y=0.465 1X+4.602 4(R2=1,P<0.001),回归得出的结果与观察评分之间存在高度吻合。结论采用本文方法可为肌力的评定提供客观依据,有望在法医鉴定实践中应用。
Objective To investigate the feasibility of using TMS-MEP to evaluate motor dysfunction after spinal cord injury in rats and to evaluate its value in forensic medicine. Methods 100 SD female rats were divided into three groups: injury group, sham operation group and normal control group. Spinal cord injury models were made by Allen’s strike device in each injury group. Motor function scores were evaluated according to the criteria of motor function of spinal cord before injury, 1 day, 1 week, 2 weeks and 4 weeks after injury. The TMS-MEP of gastrocnemius was recorded. The data were processed by SPSS 19.0 software. One-way ANOVA was used to compare the differences between groups. Results Compared with normal control group, sham operation group and experimental group, there was a significant difference (P <0.05) in injury group with the increase of striking energy, aggravation of spinal cord injury, absence of motor function of both lower extremities. The latency and amplitude of MEP in the injury group were longer than those in the normal control group, the difference was significant (P <0.05). The regression equation of TMS-MEP was Y = 0.465 1X + 4.602 4 (R2 = 1, P <0.001). The regression results were highly consistent with the observed scores. Conclusion This method can provide an objective basis for the assessment of muscle strength and is expected to be applied in forensic practice.