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目的观察系统性红斑狼疮(systematic lupus erythematosus,SLE)患者血清中25羟基维生素D3[25(OH)D3]水平变化;25(OH)D3与体内浆细胞样树突状细胞(plasmacytoid dendritic cell,pDC)数量、pDCs的表面分子及SLE疾病活动指数(SLE disease activity index,SLEDAI)相关性。方法门诊收取符合条件的SLE患者46例和14例健康对照者的血清,SLE患者按治疗分为未治疗组、单用激素组、激素加羟基氯喹组,放射免疫法测定25(OH)D3水平,流式细胞术测外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中pDCs水平和pDCs表面CD32、CD40、CD86、CD62L、CXCR4表达和改变,比较SLE患者与健康对照组、SLE不同治疗组间检测指标的差别。结果 SLE患者血清25(OH)D3水平较健康对照组低(t=3.06,P=0.003);SLE患者不同治疗组间25(OH)D3水平有统计学差异(F=4.62,P=0.015),未用药组低于激素组;25(OH)D3水平与SLE患者SLEDAI评分呈负相关(r=-0.38,P=0.050);在不同的25(OH)D3浓度范围下,pDCs的水平、SLEDAI评分差异均有统计学意义(均有P<0.05);25(OH)D3水平与3组SLE患者pDCs水平及表面分子均无明显相关性(均有P>0.05)。结论SLE患者血清25(OH)D3水平低下,25(OH)D3水平对pDCs水平及疾病活动度可能有影响,
Objective To investigate the changes of serum 25-hydroxyvitamin D3 (25 (OH) D3] in patients with systemic lupus erythematosus (SLE). 25 (OH) D3 and plasmacytoid dendritic cells ), The number of surface molecules of pDCs and the SLE disease activity index (SLEDAI). Methods The outpatients received serum from 46 healthy SLE patients and 14 healthy controls. The SLE patients were divided into untreated group, hormone group and hydroxychloroquine alone group, and the levels of 25 (OH) D3 were determined by radioimmunoassay , The level of pDCs in peripheral blood mononuclear cells (PBMC) and the expression and change of CD32, CD40, CD86, CD62L and CXCR4 in peripheral blood mononuclear cells (PBMCs) were detected by flow cytometry. Differences between test indicators. Results Serum 25 (OH) D3 levels in SLE patients were significantly lower than those in healthy controls (t = 3.06, P = 0.003). There was a significant difference in serum 25 (OH) D3 levels among different treatment groups (F = 4.62, (OH) D3 level was negatively correlated with SLEDAI score of SLE patients (r = -0.38, P = 0.050). The levels of pDCs in different concentrations of 25 (OH) D3, (P <0.05). The level of 25 (OH) D3 had no significant correlation with the levels of pDCs and surface molecules in SLE patients (all P> 0.05). Conclusions Serum 25 (OH) D3 levels are low in patients with SLE. The level of 25 (OH) D3 may have an impact on the level of pDCs and disease activity.