丝虫感染期蚴(L_3)的低温保存,国内外已有一些报道。冷冻后的L_3仍能保持原有的生物学特性,在易感宿主体内能继续发育至成虫;亦能在体外继续培养,蜕皮发育至童虫(L_4)。鉴于L_3的来源和虫种的不同,所采用的冷冻方法也各异,目前尚无标准化的方法。本实验采用不同冷冻方法,对马来丝虫L_3进行低温保存研究,以探索长时间冷冻保存L_3的实验方法。材料与方法一、虫源周期型马来丝虫(Brugia malayi)感染期蚴,从本实验室马来丝虫长爪沙鼠动物模 Filariasis infection larvae (L_3) cryogenic preservation, there have been some reports at home and abroad. Frozen L_3 can still maintain the original biological characteristics, in susceptible host body can continue to develop into adults; also continue to train in vitro, molting development to Schistosoma japonicum (L_4). Due to the different sources and species of L_3, the freezing methods used are also different. There is no standardized method. In this experiment, different freezing methods were used to study the cryopreservation of L_3 in Malayan filariasis in order to explore the experimental method of cryopreserving L_3 for a long time. Materials and methods First, the worm period Cyclophilus (Brugia malayi) infection stages of larvae, from the laboratory malayian worm Clawed animal model