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采用生物活性追踪法,从侧柏内生真菌Chaetomium globosum ZH-32发酵液中分离得到1个具有抑菌活性的化合物——WH-01,经紫外光谱、核磁共振及质谱等鉴定为球毛壳菌素A。抑菌活性测定结果表明:WH-01对枯草芽孢杆菌Bacillus subtilis、蜡状芽孢杆菌Bacillus cereus、金黄色葡萄球菌Staphylococcus aureus和大肠埃希氏菌Escherichia coli有强烈的抑制作用,其最低抑制浓度(MIC)分别为3.13、3.13、1.56和3.13μg/mL;对番茄灰霉病菌Botrytis cinerea和油菜菌核病菌Sclerotinia sclerotiorum菌丝生长的抑制中浓度(EC50)分别为5.36和12.19μg/mL。
A bioactive tracing method was used to isolate one of the compounds with antibacterial activity, HW-01, from Chaetomium globosum ZH-32 fermentation broth and identified as globular shell by ultraviolet spectroscopy, nuclear magnetic resonance and mass spectrometry A The results of antibacterial activity assay showed that WH-01 had a strong inhibitory effect on Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and Escherichia coli. The minimum inhibitory concentration (MIC ) Were 3.13, 3.13, 1.56 and 3.13 μg / mL, respectively. The inhibitory concentrations (EC50) of mycelial growth on Botrytis cinerea and Sclerotinia sclerotiorum were 5.36 and 12.19 μg / mL, respectively.