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目的探讨槐耳清膏体外逆转人肝癌细胞多药耐药性的作用及可能的机制。方法MTT法检测槐耳清膏的细胞毒作用和处理前后耐药细胞对化疗药物的敏感性;流式细胞仪检测细胞内阿霉素浓度;RT-RCR检测MDR1基因的表达。结果槐耳清膏在0.01g/L以下剂量时对HepG2和HepG2/ADM耐药细胞株的抑制率均<10%,半数抑制率(IC_(50))分别为0.917 g/L和1.66 g/L,无细胞毒剂量即0.008 g/L的槐耳清膏能部分逆转HepG2/ADM细胞的耐药性,与之合用使耐药肝癌细胞对阿霉素、顺铂、丝裂霉素、氟尿嘧啶的相对逆转效率分别为79%、73.5%、97.4%和86.7%,HepG2/ADM细胞内阿霉素浓度明显增加,MDR1表达下降了33.7%。结论槐耳清膏具有体外逆转人肝癌细胞多药耐药性的作用,可能与下调MDR1表达、增加细胞内化疗药物积累有关。
Objective To investigate the effect and possible mechanism of Huai’er Qingpao on reversing multidrug resistance in human hepatocellular carcinoma cells in vitro. Methods MTT assay was used to detect the cytotoxicity and the chemosensitivity of drug-resistant cells before and after treatment. The intracellular adriamycin concentration was detected by flow cytometry. The expression of MDR1 gene was detected by RT-PCR. Results The results showed that the inhibitory rates of HepG2 and HepG2 / ADM were both less than 10% and the IC50 of IC50 were 0.917 g / L and 1.66 g / L, respectively. L, non-cytotoxic dose of 0.008 g / L of Hua ear cream can partially reverse the drug resistance of HepG2 / ADM cells, with the combination of drug-resistant liver cancer cells doxorubicin, cisplatin, mitomycin, fluorouracil The relative reversal efficiency of HepG2 / ADM cells was 79%, 73.5%, 97.4% and 86.7% respectively. The concentration of doxorubicin in HepG2 / ADM cells was significantly increased and the expression of MDR1 was decreased by 33.7%. CONCLUSION: Huaierqing cream can reverse the multidrug resistance of human hepatocellular carcinoma cells in vitro, which may be related to the down-regulation of MDR1 expression and the increase of intracellular chemotherapeutic drug accumulation.