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采用毛细管电泳-安培法(CE-AD)同时分离测定了磺胺甲噁唑(sulfamethoxazole,SMZ)、磺胺嘧啶(sulfadiazine,SD)和抗菌增效剂甲氧苄胺嘧啶(trimethoprim,TMP)3种常用磺胺类抗菌药物成分,考察了实验参数对分离、检测体系的影响。在优化实验条件下,以300μm碳圆盘电极作为工作电极,检测电位为1050mV(vs.SCE),在Na2B4O7(13mmol/L)-KH2PO4(18mmol/L)(pH5.8)的缓冲溶液中,分离电压18kV,进样6s,3组分在14min内可实现基线分离。上述3组分浓度分别在5×10-4~5×10-2、5×10-4~0.1和5×10-4~5×10-2g/L范围内与其峰电流强度呈线性关系,检出限达5.1×10-5~8.0×10-5g/L(S/N=3)。该方法已成功应用于复方磺胺甲噁唑片中抗菌活性成分的含量测定,结果令人满意。
Three commonly used sulfamethoxazole (SMZ), sulfadiazine (SD) and antibacterial synergist trimethoprim (TMP) were determined by capillary electrophoresis with amperometry (CE-AD) Sulfa antibacterial ingredients, investigated the experimental parameters on the separation and detection system. Under optimized experimental conditions, the detection voltage was 1050mV (vs.SCE) with 300μm carbon disk electrode as the working electrode, and in the buffer solution of Na2B4O7 (13mmol / L) -KH2PO4 (pH5.8) , The separation voltage 18kV, injection 6s, 3 components within 14min to achieve baseline separation. The concentration of the above three components showed a linear relationship with the peak current intensity in the range of 5 × 10-4 ~ 5 × 10-2, 5 × 10-4 ~ 0.1 and 5 × 10-4 ~ 5 × 10-2g / L, respectively, The detection limit reached 5.1 × 10-5 ~ 8.0 × 10-5g / L (S / N = 3). The method has been successfully applied to the determination of compound sulfamethoxazole tablets antibacterial active ingredient content, the results are satisfactory.