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AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood. METHODS: Human whole blood or different type of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP-1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000μmol/L induced production of MCP-1 and IL-8 in cultured human whole blood (P<0.05). The major cellular source of these chemokines corned from monocytes. Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 μmol/L in the cultured whole blood. The intracellular ROS, particular the OH’ radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 production. CONCLUSION: Increased Hcy level in plasma (hyperhomocysteinemia) induced MCP-1 and IL-8 secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism.
METHODS: Human whole blood or different type of peripheral (AIM): To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattractant protein- 1 (MCP-1) and interleukin- 8 Results: MCP-1 and IL-8 levels were measured by ELISA assay. RESULTS: Hcy 10-1000 μmol / L induced production of MCP-1 and IL-8 in cultured human Whole blood (P <0.05). The major cellular source of these chemokines corned from monocytes. Meanwhile, Hcy also promoted the upregulation of MPO level even at the 10 μmol / L in the cultured whole blood. The intracellular ROS, radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 production. CONCLUSION: Increased Hcy level in plasma (hyperhomocysteinemia) induced MCP-1 and IL-8 secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism.