安定是苯二氮(艹卓)类的代表药物,长期应用即使是治疗剂量也能产生身体依赖性。但关于其依赖性产生的原因研究较少,本文通过研究安定依赖性小鼠肝微粒体细胞色素P_(450)和蛋白水平及血浆安定浓度的改变来探讨安定依赖产生的机理。用安定掺食法建立安定依赖性小鼠的动物模型。实验设有6组。自然戒断组和安定依赖组按掺食法递增安定剂量进食至56 d,戒断时给予普通料粉,对照组进食普通料粉至56 d。 Diazepam is the representative benzodiazepine drug, and long-term use can produce body dependence even at therapeutic doses. However, there are few researches on the causes of its dependence. In this paper, the mechanism of diazepam dependence was studied by studying the changes of cytochrome P_ (450) and protein levels in liver microsomes and plasma stability in diazepam-dependent mice. Establishment of animal model of diazepam-dependent mice by oral administration of diazepam. The experiment has 6 groups. Natural withdrawal group and stability dependence group, according to the dosages of increasing dosages of dosages to eat until 56 d, to give normal powdered sugar withdrawal, the control group fed ordinary meal to 56 d.