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以从新疆天山地区新疆野苹果600个无性系中筛选得到的18个优系为研究对象,调查其叶形性状变异,并进行了SSR分子标记分析,探究叶形性状与SSR标记间的相关关系。结果表明,调查的15个叶形性状在无性系间均存在极显著差异;平均变异系数最大的为叶尖角α(达到25.39%),叶形指数L1/L3最小(仅为10.31%);叶片长的重复力最大(达到0.967),叶形指数L1/L3最小(仅为0.495)。以15个叶片性状为依据,可将各无性系完全区分开,并分为3类,各无性系间的遗传距离变化在1.293~7.235间。用均匀分布在17个染色体连锁群上的30对多态性SSR引物对18个无性系进行聚类分析,可将各无性系完全区分开,并可分为4类,各无性系间的遗传距离为0.089~0.689,平均遗传距离为0.433。所有叶形性状聚类结果与所有SSR标记聚类结果不相关。以单一叶形性状聚类结果与经过筛选的部分SSR标记聚类结果进行相关分析,绝大部分达到极显著相关,且大部分引物组合表现为累积效应,表明叶形性状与部分SSR位点具有紧密关联。
In this study, 18 elite lines selected from 600 clones of wild apple trees in Xinjiang Tianshan Mountains were used as research objects to investigate the variation of leaf traits and SSR molecular marker analysis to explore the correlation between leaf traits and SSR markers . The results showed that there were extremely significant differences among the 15 tested leaf traits in clones. The highest average coefficient of variation was α (25.39%) and leaflet index (L1 / L3) was the lowest (only 10.31%). The leaf has the largest repetitive force (0.967) and the leaf index L1 / L3 is the smallest (only 0.495). Based on 15 leaf traits, the clones could be completely distinguished and divided into three groups. The genetic distances among clones varied from 1.293 to 7.235. Thirty ectodomain clones were clustered using 30 pairs of polymorphic SSR primers evenly distributed on 17 linkage groups. All the clones could be completely distinguished and could be divided into four groups. The genetic relationships among clones The distance is 0.089 ~ 0.689, the average genetic distance is 0.433. All leaf shape clustering results were not related to all SSR marker clustering results. Correlation analysis between single-leaf clustering results and selected SSR markers indicated that most of them were highly significant, and most of the combinations showed cumulative effects, which indicated that the leaf traits and some SSR loci had Closely linked.