论文部分内容阅读
拉曼光谱检测常常受到荧光干扰,而移频激发拉曼差分光谱法(SERDS)是一种有效抑制拉曼光谱荧光背景的方法。基于该方法,采用两个波长相近、基于体布拉格光栅技术的固定波长激光器,设计了一套拉曼光谱测试系统。通过控制激光器的功率和温度,保证了输出波长的稳定性。采用高灵敏度的薄型背照式面阵CCD,设计了光谱数据采集光路和电路。同时,在软件上实现了差分光谱的三种重构算法,即简单积分算法、带数值插值的简单积分算法和多重约束解卷积算法。在实验中,利用文中系统对强荧光背景的某品牌香油进行了拉曼光谱测量,分别采用这三种算法处理差分光谱进行光谱重构,并对比了重构效果。实验结果表明,设计的系统能够有效抑制荧光对拉曼光谱检测的影响。
Raman spectroscopy is often disturbed by fluorescence, and Raman Difference Spectroscopy (SERDS) is an effective method to suppress the Raman fluorescence background. Based on this method, a set of Raman spectroscopy test system was designed based on two fixed wavelength lasers based on Bragg grating with similar wavelengths. By controlling the laser power and temperature, to ensure the stability of the output wavelength. Using high-sensitivity thin back-illuminated area array CCD, the optical data acquisition optical circuit and circuit are designed. At the same time, three reconstruction algorithms of differential spectrum are implemented in software, namely simple integral algorithm, simple integral algorithm with numerical interpolation and multiple constrained deconvolution algorithm. In the experiment, Raman spectroscopy was performed on a brand of sesame oil with a strong fluorescence background by using the system in the article. Spectral reconstruction was performed by using these three algorithms to deal with the difference spectrum respectively, and the reconstruction effect was compared. The experimental results show that the designed system can effectively inhibit the fluorescence detection of Raman spectra.