论文部分内容阅读
为了观察肺纤维化形成过程中Ⅰ、Ⅲ型前胶原的变化，以及它在肺纤维化发生发展中的作用，我们用博莱霉素A5建立肺纤维化动物模型，第7、14、29天取肺组织用原位杂交的方法检测Ⅰ、Ⅲ前胶原的基因表达，发现两种前胶原在第7天表达至高峰，第29天Ⅰ型仍保持高水平，Ⅱ型已恢复正常。提示抑制Ⅰ型胶原，并保持Ⅲ型与Ⅰ型胶原正常比率的药物可能对防治肺纤维化有效。
In order to observe the changes of type I and type III procollagen and the role of procollagen in the development and progression of pulmonary fibrosis, we established animal models of pulmonary fibrosis with bleomycin A5. On the 7th, 14th and 29th days The lung tissues were examined by in situ hybridization for the gene expression of procollagen Ⅰ and Ⅲ. The expression of procollagen Ⅱ was peaked on the 7th day. The type Ⅰ remained high on the 29th day and the type Ⅱ returned to normal. Prompt inhibition of type I collagen, and to maintain the normal ratio of type Ⅲ and type Ⅰ collagen drugs may be effective in the prevention and treatment of pulmonary fibrosis.