China is one of the twelve prolific biological diversity countries in the world, and nematophagous fungol resources are very abundant. In this paper, the research advance of nematophagous fungi is discussed from six aspects: resources introduction, biology of nematophagous fungi, the application of biocontrol agents, fungistsis in soils, research on nematicidal metabolites and molecular biology on nematophagous fungi. Concerning Clonostachys rosea, the alkaline protease (designated Lmz1) was purified from supernatant of C. rosea which showed a molecular mass of 33 kDa, pI of pH 10.5, optimum temperature of 55- 60 ℃. and optimum pH between 11.0-12.0. N-terminal sequence of Lmz1 was determined by electoblotting enzyme protein to PVDF membrane as NH2-A-T-Q-S-N-A-P-?-?-?. The degenerate primer was designed according to terminal sequence for cDNA gene encoding mature protein of Lmz1 by 3’RACE system for rapid amplification of cDNA ends. To express successfully a fungal alkaline protease (Lmz1) in a high level expression system. The specific primers with EcoRI recognition site and extra bases were designed. The cDNA gene encoding mature protease (Lmz1) was amplified with high fidelity pfu DNA polymerase and specific primers against total cDNA population of C. rosea. The recombinant expression vector contain desired DNA fragment was correctly constructed and introduced into yeast host Pichia pastoris GS115 by electroporation. The recombinant alkaline protease was characterized by protease activity, PCR amplification against yeast total DNA, ELISA and western blotting. It demonstrated a molecular mass of 42 kDa based on blot analysis. The expression level of Lmz1 was approximately 9 mg/mL by UV spectrophotometric method. China is one of the twelve prolific biological diversity countries in the world, and nematophagous fungol resources are very abundant. In this paper, the research advance of nematophagous fungi is discussed from six aspects: resources introduction, biology of nematophagous fungi, the application of biocontrol Concerning Clonostachys rosea, the alkaline protease (designated Lmz1) was purified from supernatant of C. rosea which showed a molecular mass of 33 kDa, pI of pH 10.5, The optimum temperature of 55- 60 ° C. and the optimum pH between 11.0-12.0. N-terminal sequence of Lmz1 was determined by electoblotting enzyme protein to PVDF membrane as NH2-ATQSNAP -? -? -? sequence for cDNA gene encoding mature protein of Lmz1 by 3’RACE system for rapid amplification of cDNA ends. To express successfully a fungal alkaline pro The specific primers with EcoRI recognition site and extra bases were designed. The cDNA gene encoding mature protease (Lmz1) was amplified with high fidelity pfu DNA polymerase and specific antibodies against total cDNA population of C . rosea. The recombinant expression vector contains the DNA fragment was correctly constructed and introduced into yeast host Pichia pastoris GS115 by electroporation. The recombinant alkaline protease was characterized by protease activity, PCR amplification against yeast total DNA, ELISA and western blotting. It demonstrated a Molecular mass of 42 kDa based on blot analysis. The expression level of Lmz1 was approximately 9 mg / mL by UV spectrophotometric method.