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目的研究葡萄糖调节蛋白78(GRP78)在胃癌、慢性萎缩性胃炎及浅表性胃炎组织中的表达及临床意义。方法 RT-PCR法检测胃癌、慢性萎缩性胃炎和浅表性胃炎各25例新鲜组织中GRP78基因表达;免疫组化法检测胃癌、慢性萎缩性胃炎和浅表性胃炎各60例组织中GRP78蛋白表达。分析GRP78蛋白表达与临床病理参数间的相关性。结果胃癌组织中GRP78mRNA的表达水平高于慢性萎缩性胃炎和浅表性胃炎(1.26±0.18vs.0.89±0.25和0.29±0.09)(P<0.01)。胃癌组织中GRP78蛋白的阳性表达率亦高于慢性萎缩性胃炎和浅表性胃炎(78.3%vs.46.6%和6.7%)(P<0.01)。胃癌组织中GRP78蛋白表达与胃癌分化程度、分期、淋巴结转移等明显相关(P<0.05或P<0.01)。结论 GRP78在胃癌中呈高表达;检测GRP78的表达可能有助于预防及早期诊断胃癌。
Objective To study the expression and clinical significance of glucose regulatory protein 78 (GRP78) in gastric cancer, chronic atrophic gastritis and superficial gastritis. Methods The expression of GRP78 in 25 fresh tissues of gastric cancer, chronic atrophic gastritis and superficial gastritis was detected by RT-PCR. The expression of GRP78 protein in 60 cases of gastric cancer, chronic atrophic gastritis and superficial gastritis was detected by immunohistochemistry expression. The correlation between GRP78 protein expression and clinicopathological parameters was analyzed. Results The expression of GRP78 mRNA in gastric cancer tissues was higher than that of chronic atrophic gastritis and superficial gastritis (1.26 ± 0.18 vs.0.89 ± 0.25 and 0.29 ± 0.09) (P <0.01). The positive rate of GRP78 protein in gastric cancer was also higher than that of chronic atrophic gastritis and superficial gastritis (78.3% vs 46. 6% and 6.7%, P <0.01). The expression of GRP78 in gastric cancer tissues was significantly correlated with the differentiation, staging and lymph node metastasis (P <0.05 or P <0.01). Conclusion GRP78 is highly expressed in gastric cancer. Detecting the expression of GRP78 may be helpful in the prevention and early diagnosis of gastric cancer.