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采用分离自健康茶树叶片组织中的2株内生真菌,长枝木霉CSN-18和曲霉CSN-3,研究了单独培养和混合培养条件下培养原液与培养原液乙酸乙酯萃取液对植物病原真菌的抑制作用。与2菌株单独培养相比,混合培养对稻瘟病菌Magnaporthe grisea、茶花褐斑病菌Phyllosticta camelliaecola和茶云纹叶枯病菌Guignardia camellia等8种植物病原真菌的抑制作用明显增强。混合培养原液的乙酸乙酯萃取液对茶花褐斑病菌、稻瘟病菌和茶云纹叶枯病菌的抑制率分别为79.48%±1.46%、76.99%±0.91%和71.51%±4.93%;对茶花褐斑病菌、茶云纹叶枯病菌和稻瘟病菌孢子萌发的抑制率分别为100.00%、90.90%±2.59%和84.00%±5.29%。混合培养原液对茶花褐斑病菌菌丝生长的抑制作用也比两菌株的单独培养液有明显增强;在低浓度下,混合培养原液对茶花褐斑病菌的孢子萌发有促进作用;但浓度超过40%,则明显抑制茶花褐斑病菌孢子的萌发。
Two strains of endophytic fungi, Trichoderma longibrachiatum CSN-18 and Aspergillus CSN-3 isolated from the leaves of healthy tea plants were used to study the effects of culture broths and culture extracts of ethyl acetate extract on plant pathogens Inhibition of fungi. Compared with 2 strains alone, the mixed culture significantly inhibited the growth of eight plant pathogenic fungi, Magnaporthe grisea, Phyllosticta camelliaecola and Guignardia camellia. The inhibitory rates of ethyl acetate extracts of mixed culture solution on R. solani, Magnaporthe grisea and M. difficile were 79.48% ± 1.46%, 76.99% ± 0.91% and 71.51% ± 4.93%, respectively. The inhibitory rates of brown spot, leaf blight, and blast germ were 100.00%, 90.90% ± 2.59% and 84.00% ± 5.29%, respectively. The inhibitory effect of the mixed culture solution on the mycelial growth of the brown spot pathogen Mycophenolate pallidum was also significantly enhanced than that of the two strains. At low concentration, the mixed culture liquid had a promoting effect on the spore germination of the brown spot pathogen; However, when the concentration exceeded 40 %, Then obviously inhibited the spore germination of P. crispus.