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【目的】为了分析我国不同地区禾谷缢管蚜Rhopalosiphum padi(Linnaeus)田间种群Ace1基因的选择性剪切、Ace1基因选择性剪切频率及Ace1基因选择性剪切对其ACh E1结构的影响。【方法】从我国11个省(市)的16个地区采集田间试虫,通过RT-PCR技术,克隆各个地区及室内敏感品系各15头禾谷缢管蚜试虫的Ace1基因序列的全长,分析并鉴定Ace1的选择性剪接,在SABLE服务器和I-TASSER服务器上分别分析选择性剪切对ACh E1的二级结构和三级结构的影响。【结果】16个地理种群中,9个地理种群都存在选择性剪切,不同地理种群该选择性剪切的频率不同;剪切位点靠近Ace1基因的5′端,其对应的核苷酸序列为5′-ATCCGAATTTAG-3′,导致4个氨基酸(RSEF)缺失;选择性剪切改变了局部的二级结构,并在一定程度上改变其三级结构。【结论】禾谷缢管蚜田间种群中较为普遍地存在一个Ace1基因的选择性剪切,该Ace1基因选择性剪切改变了ACh E1的空间结构,并可能改变ACh E1的代谢特性。
【Objective】 The objective of this study was to analyze the effects of selective cleavage of Ace1 gene, selective acetylation frequency of Ace1 gene and selective acetylation of Ace1 gene on the ACh E1 structure in Rhopalosiphum padi (Linnaeus) field in different regions of China. 【Method】 The field test insects were collected from 16 areas of 11 provinces (cities) in our country. The total length of the Ace1 gene of 15 S. cerealis aphids from each region and indoor susceptible strain was cloned by RT-PCR , Analyze and identify the alternative splicing of Ace1 and analyze the effect of selective cleavage on the secondary structure and tertiary structure of ACh E1 on SABLE server and I-TASSER server, respectively. 【Result】 Nineteen geographic populations of 16 geographic populations were all selectively cut, and the frequency of selective cutting was different among different geographical populations. The cleavage site was close to the 5 ’end of Ace1 gene and its corresponding nucleotide The sequence is 5’-ATCCGAATTTAG-3 ’, leading to the deletion of four amino acids (RSEF); selective cleavage changes the local secondary structure and to some extent changes its tertiary structure. 【Conclusion】 Apoptosis of Ace1 gene is more commonly observed in the field population of S. avenae. The selective cleavage of Ace1 gene changes the spatial structure of ACh E1 and may change the metabolic characteristics of ACh E1.