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目的:研究六味地黄丸(颗粒)对哮喘小鼠糖皮质激素受体(GR)mRNA的影响。方法:应用荧光定量聚合酶链反应(FQ-PCR)法检测哮喘小鼠肺组织内GRmRNA表达的相对量,比较正常对照组、哮喘模型组、用药组之间的GRmRNA表达差异。结果:与正常组比较,其余各组的GRmRNA表达明显降低(P<0.05);各用药组与哮喘模型组相比GRmRNA表达无明显差异。结论哮喘的发病可能在一定程度上与GRmRNA降低有关,六味地黄颗粒对哮喘小鼠GRmRNA没有明显的调节作用。
Objective: To study the effects of Liuweidihuang Pills (granules) on glucocorticoid receptor (GR) mRNA in asthmatic mice. Methods: The relative amount of GR mRNA expression in the lung tissue of asthmatic mice was detected by fluorescence quantitative polymerase chain reaction (FQ-PCR). The difference of GR mRNA expression between normal control group, asthma model group, and drug group was compared. Results: Compared with the normal group, the expression of GR mRNA in the other groups was significantly lower (P<0.05). There was no significant difference in the expression of GR mRNA in each drug group compared with the asthma model group. Conclusion The onset of asthma may be related to the decrease of GR mRNA to a certain extent. Liuweidihuang granule has no obvious regulation on GR mRNA in asthmatic mice.