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目的 :探讨芦荟 (aloevera)治疗大鼠实验性变应性鼻炎的作用机制。方法 :用卵清蛋白致敏大鼠制成变应性鼻炎动物模型 ,将芦荟经鼻给药 ,观察其行为学差异 ;用组织病理学方法观察鼻粘膜改变 ;用逆转录 多聚酶链反应 (RT PCR)对鼻粘膜和脾组织IL 2 ,IL 4mRNA进行定量检测。结果 :行为学得分 ,阳性对照组[(8.4 2± 1.0 6 )分 ]明显高于实验组 [(2 .0 2± 0 .4 2 )分 ]和正常对照组 (0分 ) ,其差异有极显著性意义 (P <0 .0 1) ;实验组鼻粘膜炎性反应明显轻于阳性对照组 ;实验组IL 2mRNA表达水平明显高于阳性对照组 ,其差异有极显著性意义 (P <0 .0 1) ;实验组IL 4mRNA表达水平明显低于阳性对照组 ,其差异也有极显著性意义 (P <0 .0 1)。结论 :芦荟通过改变TH1和TH2细胞因子基因表达 ,调节CD4+ 淋巴细胞亚群分化而发挥治疗变应性鼻炎的作用
Objective: To investigate the mechanism of action of aloevera on experimental allergic rhinitis in rats. Methods: Rat model of allergic rhinitis was made by ovalbumin sensitization. Nasal administration of Aloe vera was performed to observe the difference of behavior. The nasal mucosa was observed by histopathology. PCR) quantitative detection of IL 2, IL 4 mRNA in nasal mucosa and spleen. Results: The behavioral score was significantly higher in the positive control group (8.42 ± 1.06) than in the experimental group (2.02 ± 0.42) and in the normal control group (0) (P <0.01). The inflammatory reaction of nasal mucosa in the experimental group was lighter than that of the positive control group. The level of IL-2 mRNA in the experimental group was significantly higher than that in the positive control group (P < 0. 01). The level of IL 4 mRNA in the experimental group was significantly lower than that in the positive control group (P <0.01). Conclusion: Aloe vera exerts the therapeutic effect on allergic rhinitis by altering TH1 and TH2 cytokine gene expression and regulating CD4 + lymphocyte subsets differentiation