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目的研究5-羟色胺转运体渊SERT冤与肠易激综合征渊IBS冤患者躯体和心理症状是否存在相关性。方法 选取2010年1月~2012年12月佛山市第一人民医院IBS患者154例为病例组,并按不同症状分为3个亚组(C-IBS组65例、D-IBS组47例、A-IBS组42例),选择同期健康志愿者154名作为对照组。应用心理症状自评量表(SCL-90)来评价研究对象的心理状态;采用实时定量PCR法测定SERT信使RNA(mRNA)表达量袁Western印迹法测定SERT蛋白表达水平。结果 SERT蛋白表达量:病例组(0.81±0.45)和对照组(0.79±0.47)比较,差异无统计学意义(P>0.05)。SERT mRNA转录表达量:病例组(0.46±0.25)和对照组(0.42±0.28)比较,差异无统计学意义(P>0.05)。C-IBS组SERT蛋白表达量(0.98±0.51)及mRNA表达量(0.58±0.23)均显著高于D-IBS组[(0.71±0.40)、(0.41±0.25)]、A-IBS组[(0.67±0.35)、(0.37±0.23)]和对照组[(0.79±0.47)、(0.42±0.28)],差异均有统计学意义(P<0.05)。SERT蛋白表达量与躯体化症状(r=-0.681,P<0.05)、敌意因子(r=-0.663,P<0.05)、焦虑因子(r=-0.711,P<0.05)和抑郁因子(r=-0.812,P<0.05)呈负相关,与强迫症状、恐惧、偏执、人际关系敏感和精神病因子均无明显相关性(P>0.05)。结论 IBS患者SERT的mRNA及蛋白量表达水平与患者的躯体和心理症状密切相关,越低则越容易出现躯体化症状、焦虑等情绪表现。
Objective To investigate whether there is a correlation between the somatic and psychological symptoms of serotonergic transporter SERT and IBS in patients with IBS. Methods A total of 154 patients with IBS in Foshan First People’s Hospital from January 2010 to December 2012 were selected as case group and divided into three subgroups according to different symptoms (65 cases in C-IBS group, 47 cases in D-IBS group, A-IBS group 42 cases), select the same period 154 healthy volunteers as a control group. The mental state of the subjects was evaluated by using SCL-90. The expression of SERT mRNA was detected by real-time PCR. The expression of SERT protein was detected by Western blotting. Results The expression of SERT protein showed no significant difference between the case group (0.81 ± 0.45) and the control group (0.79 ± 0.47) (P> 0.05). SERT mRNA transcript levels: There was no significant difference between the case group (0.46 ± 0.25) and the control group (0.42 ± 0.28) (P> 0.05). The expression of SERT protein (0.98 ± 0.51) and mRNA (0.58 ± 0.23) in C-IBS group were significantly higher than those in D-IBS group (0.71 ± 0.40, 0.67 ± 0.35), (0.37 ± 0.23) and control group (0.79 ± 0.47) and (0.42 ± 0.28), respectively. There was significant difference between the two groups (P <0.05). The expression of SERT protein was associated with somatization (r = -0.681, P <0.05), hostility (r = -0.663, P <0.05), anxiety (r = -0.711, -0.812, P <0.05). There was no significant correlation between obsessive compulsive symptoms, fear, paranoid, interpersonal sensitivity and psychotic factors (P> 0.05). Conclusion The expression level of SERT mRNA and protein in patients with IBS is closely related to somatic and psychological symptoms of patients. The lower the degree of SERT, the more likely it is to manifest somatic symptoms and anxiety.