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用３Ｈ－胸腺嘧啶脱氧核苷掺入法研究了蛙皮素（ｂｏｍｂｅｓｉｎ，ＢＯＭ）及其受体拮抗剂［Ｄ－Ａｒｇ１，Ｄ－Ｐｈｅ５，Ｄ－Ｔｒｐ７，９，Ｌｅｕ１１］－ｓｕｂｓｔａｎｃｅＰ（ＢＯＭ－Ａ）对人胃癌细胞株ＳＧＣ７９０１生长的影响，并通过测定细胞内三磷酸肌醇（ＩＰ３）含量，进一步观察蛙皮素对胃癌细胞的受体后细胞内信息传递途径。结果示：蛙皮系能明显促进人胃癌细胞株的生长，而蛙皮素受体拮抗剂［Ｄ－Ａｒｇ１，Ｄ－ｐｈｅ５，Ｄ－Ｔｒｐ７，９，Ｌｅｕ１１］－ｓｕｂｓｔａｎｃｅＰ，能抑制蛙皮素对胃癌细胞的促生长作用。在无血清培养基中，上述受体拮抗剂对人胃癌细胞生长也有直接的抑制，提示蛙皮素对胃癌细胞有自分泌调节作用。蛙皮素对细胞生长的促进作用是通过磷酸肌醇途径，使细胞内ＩＰ３增加所致。
Bombaresin (BOM) and its receptor antagonist [D-Arg1,D-Phe5,D-Trp7,9,Leu11]-substanceP (BOM-A) were studied by 3H-thymidine incorporation method. ) Effect on the growth of human gastric cancer cell line SGC7901, and by measuring intracellular inositol triphosphate (IP3) content, further observe the intracellular information transmission pathway of bombesin on the receptor of gastric cancer cells. The results showed that the frog skin system can significantly promote the growth of human gastric cancer cell lines, while the bombesin receptor antagonist [D-Arg1, D-phe5, D-Trp7, 9, Leu11]-substanceP can inhibit the The role of gastric cancer cells in promoting growth. In serum-free medium, the above-mentioned receptor antagonists also directly inhibit the growth of human gastric cancer cells, suggesting that there are autocrine-modulating effects of bombesin on gastric cancer cells. Bombesin promotes cell growth through the phosphoinositide pathway, which results in increased intracellular IP3.