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目的了解福建省宁德市少数民族地区结核分枝杆菌的基因型特征。方法采用基于IS6110的PCR分型方法,对宁德市少数民族地区的73株结核分支杆菌进行检测,用Gel-Pro analyzer4.0软件和NTSYSpc2.10e软件对DNA指纹图谱进行聚类分析。结果根据IS6110-PCR指纹图谱,73株结核分支杆菌共分为两个主要类型,其中Ⅰ型48株占65.8%,Ⅱ型(北京基因型)25株占34.2%。汉、畲两族病例菌株指纹均为Ⅰ、Ⅱ型,但在两型中的分布差异有显著性(P<0.01),畲族以Ⅰ型居多,占78.9%。I、Ⅱ型菌株耐药率分别为39.6%(19/48)、28.0%(7/25)。结论宁德市少数民族地区结核分支杆菌北京基因型呈一般水平的流行,汉、畲两族之间结核病存在近期传播关系,但畲族人群病例以Ⅰ型(非北京基因型)流行为主,且耐药率较高。
Objective To understand the genotypes of Mycobacterium tuberculosis in Ningde minority in Fujian province. Methods 73 strains of Mycobacterium tuberculosis in Ningde minority area were detected by IS6110-based PCR typing method. DNA fingerprinting was performed using Gel-Pro analyzer 4.0 software and NTSYSpc2.10e software. Results According to the IS6110-PCR fingerprinting, 73 strains of Mycobacterium tuberculosis were divided into two major types, of which 48 strains were type Ⅰ (65.8%) and 25 (type Ⅱ) (Beijing) were 34.2%. The fingerprints of both Han and She strains were type I and type II, but there was a significant difference in distribution between the two types (P <0.01). Type I was predominant in She population, accounting for 78.9%. The drug resistance rates of type I and type II strains were 39.6% (19/48) and 28.0% (7/25), respectively. Conclusions Beijing genotypes of Mycobacterium tuberculosis in Ningde minority areas are generally epidemic. There is a recent relationship between tuberculosis prevalence in Han and She nationalities. However, the prevalence of type Ⅰ (non-Beijing genotypes) prevalence in She population is high Drug rates higher.