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本文以生长指数、绿原酸类物质含量和每升培养基中绿原酸的产量为评价标准,研究了培养基种类、碳源、蔗糖浓度、光照条件及水解酪蛋白(CH)浓度对黄栀子愈伤组织的生长及其次级代谢产物绿原酸类物质积累的影响,旨在为生产绿原酸类物质提供依据。研究显示,培养条件对黄栀子愈伤组织生长状况和绿原酸类物质积累有显著影响,适合生长的培养条件与适合绿原酸类物质积累的条件并不一致;适合黄栀子愈伤组织生长的最佳培养条件为:以MS为基础培养基,麦芽糖+蔗糖为碳源、糖浓度为3%、半光照条件下鲜重增长指数最大,达到47.11%。适合绿原酸类物质积累使得总产量达到最大的最佳培养条件为:MS培养基、蔗糖为碳源、蔗糖浓度为5%、不添加水解酪蛋白、半光照条件下培养,最高产量达每升培养基含35.55 mg绿原酸类物质。
In this paper, the growth index, the content of chlorogenic acids and the yield of chlorogenic acid per liter of culture medium were used as evaluation criteria. The effects of medium type, carbon source, sucrose concentration, light conditions and hydrolyzed casein (CH) Gardenia callus growth and secondary metabolites of chlorogenic acid accumulation of substances, aims to provide the basis for the production of chlorogenic acids. The results showed that the culture conditions had significant effects on the growth status and the accumulation of chlorogenic acids in the genus Gardenia jasminoides, and the culture conditions suitable for the growth were inconsistent with the conditions suitable for the accumulation of the chlorogenic acids. The best growth conditions were as follows: MS medium supplemented with maltose + sucrose as carbon source, the concentration of sugar was 3%, and the fresh weight growth index reached the highest under semi-light condition, reaching 47.11%. The optimum culture conditions for the accumulation of chlorogenic acids to maximize the total yield were as follows: MS medium, sucrose as carbon source, sucrose concentration of 5%, hydrolyzed casein, The culture medium contains 35.55 mg of chlorogenic acids.