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研究发现 Ce( )氧化含巯基 ( -SH)的化合物能产生弱发光 ,且 Rh6G能大大增强此弱发光 ,由此建立了测定头孢类 β-内酰胺类抗生素的 FIA-CL分析法。在选定的最佳条件下 ,经测试 ,几个典型的头孢类抗生素化学发光强度均在 1 .0× 1 0 - 8～1 .5× 1 0 - 5g/ m L范围内与其浓度呈良好的线性关系。头孢哌酮钠和头孢拉定的化学发光特性具有突出的差异
It has been found that the oxidation of thiol (-SH) -containing compounds by Ce () can produce weak luminescence, and Rh6G can greatly enhance the weak luminescence. Therefore, the FIA-CL assay for the determination of cephalosporin β-lactam antibiotics has been established. Under the optimal conditions, the chemiluminescence intensities of several typical cephalosporins were tested at concentrations ranging from 1.0 × 10 -8 to 1.5 × 10 5 g / m L The linear relationship. Cefoperazone sodium and cephradine chemiluminescence characteristics with outstanding differences