本实验用ＰＣＲ、ＰＣＲ－ＳＳＣＰ银染方法对２４例血清ＨＢｓＡｇ（＋）患者肝癌及癌旁组织中ＨＢｘ基因表达和ｐ５３基因突变进行了检测。肝癌组织中ＨＢｘ基因表达率为１７％（４／２４），ｐ５３基因突变率为３３％（８／２４），癌旁正常组织和硬化组织中均无ＨＢｘ基因表达和ｐ５３基因突变。存在ＨＢｘ表达的肝癌组织中ｐ５３基因突变率为１００％（４／４），ｐ５３基因突变与ＨＢｘ基因表达具有相关性（Ｐ＜０．０１），提示ｐ５３基因突变机制与ＨＢＶ感染与整合以及ＨＢｘ的启动有一定关系 In this study, HBx gene expression and p53 gene mutations were detected in 24 cases of serum HBsAg(+) patients with hepatocellular carcinoma and paracancerous tissues by PCR and PCR-SSCP silver staining. The expression rate of HBx gene in hepatocellular carcinoma was 17% (4/24), and the mutation rate of p53 gene was 33% (8/24). No HBx gene expression and p53 gene mutation were found in normal tissue and hardened tissue adjacent to cancer. The mutation rate of p53 gene in HCC tissues with HBx expression was 100% (4/4). The p53 gene mutation was associated with HBx gene expression (P<0.01), suggesting p53 gene mutation mechanism and HBV infection and integration, and HBx The start has a certain relationship