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目的观察系膜增生性肾小球肾炎患者血清Th1和Th2类细胞因子(INF-γ、IL-4、IL-10)表达变化。方法采用酶联免疫吸附实验(ELISA)测定10例正常人和25例慢性肾小球疾病患者(轻中度系膜增生性非IgA肾小球肾炎)外周静脉血血清INF-γ、IL-4、IL-10细胞因子水平。结果系膜增生性肾小球肾炎患者血清INF-γ分泌水平较正常人明显增多(P<0.01),而IL-10分泌水平较正常人明显减少(P<0.05),IL-4分泌水平则与正常人无显著差异(P>0.05),系膜增生性肾小球肾炎患者INF-γ/IL-4、INF-γ/IL-10值均显著高于正常人(P<0.05)。结论系膜增生性肾小球肾炎患者在外周血细胞因子水平存在Th1/Th2免疫失衡,可能参与了系膜增生性肾小球肾炎的免疫发病机制。
Objective To investigate the changes of serum Th1 and Th2 cytokines (INF-γ, IL-4, IL-10) in patients with mesangial proliferative glomerulonephritis. Methods Serum levels of INF-γ and IL-4 in 10 normal subjects and 25 patients with chronic glomerular disease (mild to moderate mesangial proliferative non-IgA glomerulonephritis) were measured by enzyme-linked immunosorbent assay (ELISA) , IL-10 cytokine levels. Results Serum levels of INF-γ in patients with mesangial proliferative glomerulonephritis increased significantly (P <0.01), while the levels of IL-10 secreted in patients with mesangial proliferative glomerulonephritis decreased significantly (P <0.05). The levels of IL-4 secreted The levels of INF-γ / IL-4 and INF-γ / IL-10 in patients with mesangial proliferative glomerulonephritis were significantly higher than those in normal controls (P <0.05). Conclusions Th1 / Th2 immune imbalance exists in peripheral blood cytokines in patients with mesangial proliferative glomerulonephritis, which may be involved in the immunopathogenesis of mesangial proliferative glomerulonephritis.