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目的:观察未成熟大鼠睾丸单侧扭转后对健侧睾丸血流供应和组织学的影响,并比较不同处理方法的疗效。方法:建立Wistar3周龄大鼠左侧睾丸扭转模型,分别建立对照组、扭转组、扭转复位组和扭转切除组,每组10只。彩色多普勒测量各组术前、术后8h(即扭转复位或切除术后2h)、12h、24h、72h右侧睾丸动脉收缩期最大血流速度,并于对照组和扭转组术后2h,扭转复位组和扭转切除组第1次术后12h各取2只大鼠右侧睾丸进行组织病理学观察。各组喂养至9周龄时分别取右侧睾丸进行组织学观察及检测各组大鼠右侧睾丸的生精小管直径(STD)、生精上皮细胞计数(CMSE)和睾丸活检评分(TBS)。结果:①未成熟睾丸左侧扭转后,右侧睾丸的血供呈持续性增加。②扭转组、扭转复位组和扭转切除组右侧睾丸均有不同程度的间质水肿和超微结构改变。③9周龄时扭转组、扭转切除组右侧睾丸重量均较对照组显著增加(P<0.01);各组大鼠STD、CMSE、TBS均无显著性差异(P>0.05)。结论:未成熟大鼠睾丸单侧扭转后可引起对侧睾丸的血供增加和组织学改变,轻微损伤后扭转复位和睾丸切除预后效果相似。
OBJECTIVE: To observe the effects of unilateral testicular torsion on the supply and histology of contralateral testes and to compare the curative effects of different treatment methods. Methods: The left testes torsion model was established in Wistar rats of 3 weeks old. The control group, torsion group, torsion reduction group and torsion resection group were established respectively, with 10 rats in each group. Color Doppler preoperative and postoperative 8h (ie, 2h after reversion or resection), 12h, 24h, 72h on the right side of the testis artery systolic maximum flow velocity, and in the control group and the reverse group after 2h , Reverse the reset group and the twisting resection group after the first 12h each 2 rats right testes for histopathological observation. The rats in each group were fed to 9 weeks of age, right testes were taken for histological observation and testicular spermicroscope diameter (STD), spermatogenic epithelial cell count (CMSE) and testis biopsy score (TBS) . Results: (1) The left testes of the immature twisting, the right testes blood supply showed a sustained increase. ② The torsion group, the torsion reduction group and the right testes in the torsion resection group all had different degrees of interstitial edema and ultrastructural changes. ③ At 9 weeks of age, the weight of testis in right torsion group and torsion group were significantly higher than those in control group (P <0.01). There was no significant difference in STD, CMSE and TBS between the two groups (P> 0.05). Conclusion: Unilateral unilateral testicular torsion can cause contralateral testicular blood supply and histological changes, slightly damaged after torsion reduction and orchiectomy prognosis similar.