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目的:探讨非小细胞肺癌(NSCLC)癌组织诱导型一氧化氮合酶(iNOS)的表达及其与肿瘤细胞增殖的关系。方法:采用免疫组织化学SP法对64例非小细胞肺癌术后石蜡固定标本及12例正常肺组织标本中iNOS及Ki-67抗原的表达情况进行检测,观察iNOS的表达结果与NSCLC临床病理特征及细胞增殖活性之间的关系。结果:NSCLC中的iNOS表达阳性率为81.25%,正常肺组织中无iNOS表达,iNOS的表达程度与NSCLC的病理类型、分化程度、PTNM分期、肿瘤直径、吸烟与否无关,与淋巴结转移情况呈正相关;Ki-67抗原在正常肺组织中呈低表达,在NSCLC中呈高表达,其表达强度与NSCLC的病理类型、吸烟与否无关,与分化程度、PTNM分期、肿瘤直径、淋巴结转移情况呈正相关;NSCLC癌组织iNOS表达水平与Ki-67抗原表达强度呈正相关关系。结论:NSCLC中iNOS高表达与其生长、淋巴结转移有密切关系,检测其表达情况可预示病情进展;Ki-67抗原的表达强度能够很好的反映癌细胞的增殖活性,可作为判断恶性程度、转移的客观指标;癌细胞中iNOS诱导产生的NO对肺癌细胞的增殖有促进作用。
Objective: To investigate the expression of inducible nitric oxide synthase (iNOS) in non-small cell lung cancer (NSCLC) and its relationship with tumor cell proliferation. Methods: The expression of iNOS and Ki-67 in paraffin-embedded specimens of 64 non-small cell lung cancer tissues and 12 normal lung tissues were detected by immunohistochemical SP method. The expression of iNOS and the clinicopathological features of NSCLC were observed And cell proliferation activity. Results: The positive rate of iNOS expression in NSCLC was 81.25% and no iNOS expression was found in normal lung tissue. The expression of iNOS was not related to the pathological type, differentiation, PTNM stage, tumor diameter, smoking or not, and lymph node metastasis in NSCLC The expression of Ki-67 antigen was low in normal lung tissue and high in NSCLC. The expression intensity of Ki-67 antigen was not related with the pathological type and smoking of NSCLC, but positively correlated with the degree of differentiation, PTNM stage, tumor diameter and lymph node metastasis The expression of iNOS in NSCLC was positively correlated with the expression of Ki-67 antigen. Conclusion: The high expression of iNOS in NSCLC is closely related to its growth and lymph node metastasis. Detecting the expression of iNOS in NSCLC can predict the progression of the disease. The expression of Ki-67 antigen can well reflect the proliferation activity of cancer cells and can be used as a marker of malignancy and metastasis Objective indicators; iNOS-induced NO production in cancer cells can promote the proliferation of lung cancer cells.