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目的通过对来自云南省鼠疫自然疫源地155株鼠疫菌进行基因分型,了解鼠疫菌的进化规律。方法根据22个差异区段(differentregions,DFR)设计引物,PCR扩增鼠疫菌的每个DFR。结果滇西山地闽广沿海居民区黄胸鼠鼠疫自然疫源地137株鼠疫菌的基因型均为9型。滇西山地齐氏姬鼠大绒鼠鼠疫自然疫源地18株鼠疫菌,有10株菌为7型,8株菌为9型。结论滇西山地齐氏姬鼠大绒鼠鼠疫自然疫源地鼠疫菌的基因型为7型和9型,而滇闽广沿海居民区黄胸鼠鼠疫自然疫源地鼠疫菌的基因型主要为9型。两疫源地鼠疫菌在遗传关系上有亲缘性,后者可能由前者进化而来。
Objective To understand the evolution of Yersinia pestis by genotyping 155 strains of Yersinia pestis from the natural foci of plague in Yunnan Province. Methods Based on 22 different regions (DFRs), primers were designed and PCR amplified each DFR of Y. pestis. Results The genotypes of 137 strains of Yersinia pestis from the natural foci of the raccoon dog in the coastal areas of Fujian and Guangxi in mountainous areas in western Yunnan were all 9. Eighteen strains of Yersinia pestis were found in the natural foci of plague zodiac in west mountainous area of western Yunnan, including 10 strains of 7 strains and 8 strains of 9 strains. Conclusion The genotypes of Yersinia pestis from the natural foci of natural plague in the mountainous areas of western Yunnan are Apatite 7 and 9, 9 type. Yersinia pestis of two foci in the genetic relationship has affinity, the latter may evolved from the former.