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目的制备和评估橡皮筋挂线手术方式的大动物肛瘘模型。方法选择15头猪(体质量35~42 kg)随机数字表法分成两组,实验组(8头)全麻下用橡皮筋挂线手术方法建立24个肛瘘瘘管管道,对照组(7头)全麻下用人工损伤方法建立21个管道。30 d后,所有动物进行MRI检查,实验组取出橡皮筋,两组接受临床和组织病理学评估。结果实验组与对照组在体质量、性别、瘘管数量等一般特征上对比差异无统计学意义(P>0.05)。实验后所有动物均存活。实验组24个瘘管中,瘘管不愈合者(符合临床肛瘘者)18个,经核磁共振证实在高信号状态下可清晰呈现典型瘘道结构形态者15个,经病理学确认固定的瘘管样结构者14个,临床造模成功率75.00%。对照组21个瘘管中,15个管道完全愈合,6个不愈合(符合临床肛瘘者),经核磁共振证实在高信号状态下可清晰呈现典型瘘道结构形态者5个,经病理学确认固定的瘘管样结构者4个,临床造模成功率28.57%。造模成功率比较,实验组显著优于对照组(P<0.05)。结论橡皮筋挂线手术方法建立肛瘘模型稳定可靠,磁共振成像及组织学证实,实验组挂线手术方法建立的大动物瘘管具有出丰富的肉芽组织,大动物肛瘘模型可以进一步用于研究肛瘘的诊断与治疗领域。
Objective To prepare and evaluate a large animal model of anal fistula using rubber banding technique. Methods Fifteen pigs (body weight 35-42 kg) were randomly divided into two groups. Twenty-four fistulas and fistulas were established in the experimental group (n = 8) under general anesthesia by rubber banding. The control group (n = Under general anesthesia with artificial injury method to establish 21 pipeline. After 30 days, all animals were examined by MRI, and the experimental group took rubber bands. Both groups were evaluated clinically and histopathologically. Results There was no significant difference in the general characteristics between the experimental group and the control group in terms of body weight, sex and the number of fistulas (P> 0.05). All animals survived the experiment. Experimental group of 24 fistula, fistula nonunion (in line with clinical anal fistulae) 18, confirmed by nuclear magnetic resonance in high signal state can clearly show the typical fistula structure morphology 15, confirmed by the pathological fixed fistula-like structure 14, clinical modeling success rate of 75.00%. Fifteen of the 21 fistulas in the control group were completely healed and 6 were unhealed (in line with clinical anal fistulae). Five of the typical fistula morphological structures were confirmed by MRI in the high signal state, and confirmed by pathology Fistula-like structure of four, clinical success rate of 28.57%. The success rate of modeling compared with the experimental group was significantly better than the control group (P <0.05). Conclusion The model of anal fistula established by the rubber banding method is stable and reliable. The MRI and histology confirmed that the fistula of the large animal established by the experimental group hangover surgery has rich granulation tissue. The model of large animal anal fistula can be further used to study the anal fistula Diagnostic and therapeutic areas.