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利用荧光显微镜观察太平洋牡蛎正常卵子与雄核发育卵子在受精过程、减数分裂和卵裂早期中的核相变化。雄核发育单倍体是将强度为2.8mW.cm-2.s-1的紫外线照射30s的卵子与正常精子受精后得到的。结果表明,尽管紫外线照射并没有影响卵子的成熟分裂及雌性、雄性原核的形成,但使它们的发生过程滞后。在第1卵裂中期,雄核发育卵子中雌性原核并不像雄性原核一样形成染色体,而是形成1个浓缩的染色质小体(DCB)。第1卵裂后期,DCB不参与核分裂。第1卵裂结束时,DCB位于2个分裂球其中之一的细胞质内或在赤道板处被分割成2部分。实验结果首次提供了太平洋牡蛎雄核发育的细胞学证据。
Fluorescence microscopy was used to observe the nuclear phase changes in fertilization, meiosis and early cleavage of ova in normal and ovoid eggs of Pacific oyster. The male developmental haploid is obtained by fertilizing an ovum that has been exposed to UV light of intensity 2.8 mW.cm-2.s-1 for 30 s with normal sperm. The results showed that although UV irradiation did not affect the maturation of ovum and the formation of female and male pronuclei, they lagged their development. In the middle of the first cleavage, the female pronuclei do not form the same chromosome as the male pronuclei but instead form a condensed chromatin body (DCB). After the first cleavage, DCB does not participate in mitosis. At the end of the first cleavage, the DCB is located in the cytoplasm of one of the two blastomeres or is divided into two at the equatorial plate. For the first time, the experimental results provide cytological evidence of the male nucleus of Pacific oyster.