试验探讨了利用农杆菌介导法把葡萄糖氧化酶基因（ＧＯ基因）转化到棉花中影响转化和抗性愈伤组织诱导的一些因素。结果表明，５～７ｄ 苗龄的无菌苗是做转化的最佳时期，以３Ｄ＋ ２Ｌ处理的无菌苗效果好。下胚轴的形态学部位对转化的影响差异不大。菌株的活力状态是影响转化出愈率的主要因素之一。以菌株的培养时间为依据简便有效。试验还发现，培养基的固化程度不同，出愈率有很大差异，固化程度高，异常愈伤组织增多，正常愈伤组织的出愈率降低。这可能与其活性氧平衡被打破对细胞造成伤害有关。对坑性愈伤组织的筛选次数也做了讨论。 Some factors affecting transformation and resistant callus induction by using Agrobacterium-mediated transformation of glucose oxidase gene (GO gene) into cotton were discussed. The results showed that the sterile seedlings of 5 ~ 7d seedling age were the best period for transformation, and the sterile seedlings treated with 3D + 2L had good effect. Hypocotyl morphological sites on the conversion of little difference. The viability of the strain is one of the main factors affecting the rate of conversion. Strain culture time based on simple and effective. The experiment also found that the curing degree of the medium is different, the recovery rate is very different, a high degree of curing, abnormal callus increased, the callus normal callus rate decreased. This may be related to its balance of active oxygen is broken on the cell damage caused. The number of pit callus screening was also discussed.