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目的 了解红霉素对肺纤维化的防护作用。方法 采用大鼠油酸尾静脉注射制作急性肺损伤后肺纤维化模型 ,观察红霉素对损伤肺组织中中性粒细胞聚集及氧自由基的干预作用。结果 血清检测 :油酸注射后第 12天和第 2 4天的MDA明显升高 ,而SOD明显降低 ,MDA和SOD呈负相关 (r =- 0 .91) ;红霉素干预后GSH明显降低 ;注射油酸组GSH -Px水平最高 ,红霉素干预后GSH -PX水平最低。组织匀浆检测 :MDA变化同血清 ;SOD、GSH组间无差异 ;油酸注射后第 12天和第 2 4天的GSH -Px水平明显低于红霉素干预组。镜下观察 :注射油酸组中性粒细胞浸润和纤维化程度明显重于红霉素干预组。结论 红霉素可通过抑制中性粒细胞而减少氧自由基的产生 ,减轻纤维化程度 ,对油酸所致急性肺损伤后的肺纤维化有一定防护作用
Objective To understand the protective effect of erythromycin on pulmonary fibrosis. Methods A rat model of pulmonary fibrosis induced by acute oleic acid injection was made by intravenous injection of oleic acid into the tail vein to observe the effect of erythromycin on the accumulation of neutrophils and oxygen free radicals in injured lung tissue. Results Serum levels of MDA increased significantly on the 12th and 24th days after oleic acid injection, while SOD decreased significantly (P <0.05), while MDA and SOD were negatively correlated (r = -0.91) ; The level of GSH-Px was the highest in oleic acid injection group and the lowest was GSH-PX level after erythromycin intervention. Tissue homogenate test: the change of MDA was the same as that of serum; there was no difference between SOD and GSH groups; the level of GSH-Px on the 12th day and the 24th day after oleic acid injection was significantly lower than that of erythromycin intervention group. Microscopically observed: neutrophil infiltration and fibrosis in oleic acid group were significantly higher than those in erythromycin intervention group. Conclusion Erythromycin can reduce the production of oxygen free radicals by inhibiting neutrophils, reduce the degree of fibrosis and protect the pulmonary fibrosis after acute lung injury induced by oleic acid