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背景 多成分单采是血液成分制备新一代方法,可缩短标准全血采集和制备之间的间隔时间。研究设计和方法 设计出一种单采机可较方便地同时采集出1单位(250ml)红细胞和1大容积单位(475ml)血浆。该方法使用8％ACD-A,并在欧洲两个血液中心进行试采。每个中心进行10次采集,体外评估采集RBCs和血浆;进行10次采集,体内评估RBCs恢复率。所有RBCs均用滤器减少白细胞。一半RBCs保存在Adsol添加剂溶液中,另一半保存在其它类似溶液(红细胞保存液)中。结果 在单针采集程序中,使用了3至6个循环、27分钟(范围:20～44分钟)即获得了预定体积的RBCs和血浆,盐水(275ml)用于补偿多于捐献标准全血的流体量。除轻微的血钙过低症状外,无其他副作用发生。RBC ATP在保存期间仍较好维持(42天>
Background Multi-component apheresis is a new generation of blood component preparation methods that shortens the time between standard whole blood acquisition and preparation. Study Design and Method A single machine was designed to more conveniently collect 1 unit (250 ml) of red blood cells and 1 large volume unit (475 ml) of plasma simultaneously. The method uses 8% ACD-A and is tested in two European blood centers. 10 acquisitions per center, RBCs and plasma acquisition assessed in vitro, 10 acquisitions, and in vivo assessment of RBCs recovery rates. All RBCs use filters to reduce leukocytes. Half of the RBCs were stored in Adsol additive solution and the other half in other similar solutions (red blood cell stock solution). Results In a single needle procedure, 3 to 6 cycles were used, 27 minutes (range 20 to 44 minutes) to obtain a predetermined volume of RBCs and plasma, saline (275 ml) to compensate for more than the donation of standard whole blood Fluid volume. No side effects other than mild hypocalcaemia. RBC ATP remained good during storage (42 days>