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目的研究慢性疲劳综合征基本病机,探讨升阳益胃颗粒抗疲劳的作用机制。方法雄性大鼠60只,随机分为正常组、模型组、诺迪康胶囊组、升阳益胃颗粒低、中、高剂量组,每组10只。正常组不造模,其余5组造模,时间14d。造模结束后,每组给予相应药物剂量灌胃,1次/d,连续14d。造模前、造模后以及末次灌胃后测量一次大鼠体重及力竭游泳时间。大鼠麻醉后取其股四头肌,运用ELISA法测定PGC-1α和COX的含量。结果造模后,各造模组大鼠体重及力竭游泳时间低于正常组。灌胃后,模型组大鼠体重、力竭游泳时间及PGC-1α和线粒体COX含量明显低于其余5组;正常组高于诺迪康胶囊组和升阳益胃颗粒低剂量组,有显著性差异,而与升阳益胃颗粒中、高剂量组相比,则无显著性差异;升阳益胃颗粒中剂量组与升阳益胃颗粒高剂量组相比,无明显差异。结论升阳益胃颗粒可以明显改善慢性疲劳大鼠的疲劳状态,提高骨骼肌PGC-1α和线粒体COX的含量,这可能是其抗疲劳机制之一;升阳益胃颗粒中剂量即人体常规等效剂量,增加剂量其抗疲劳疗效并不会随之增加。
Objective To study the basic pathogenesis of chronic fatigue syndrome and explore the mechanism of anti-fatigue of Shengyang Yiwei granule. Methods Sixty male rats were randomly divided into normal group, model group, Nuodikang capsule group, low, middle and high dosage of Shengyang Yisweijing granule group, with 10 rats in each group. The normal group is not model, the remaining 5 models, time 14d. After modeling, each group given the corresponding drug dose gavage, 1 / d, continuous 14d. Before modeling, after modeling and the last gavage after a rat weight and exhaustive swimming time. The rats were anesthetized to take their quadriceps muscle, using ELISA assay PGC-1α and COX content. Results After modeling, the body weight and exhausted swimming time in each model group were lower than those in normal group. After intragastric administration, body weight, exhaustive swimming time and the content of PGC-1α and mitochondrial COX in the model group were significantly lower than those in the other 5 groups. In the normal group, the body weight, There was no significant difference between the treatment group and the control group, and there was no significant difference between the treatment group and the control group. Conclusion Shengyang Yiwei granule can obviously improve the fatigue state of chronic fatigue rats and increase the content of PGC-1α and mitochondrial COX in skeletal muscle, which may be one of its anti-fatigue mechanism; Effective dose, increase the dose of its anti-fatigue effect does not increase.