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目的：探讨色素上皮衍生因子（PE DF）对高浓度葡萄糖作用下体外培养大鼠视网膜Müller细胞损伤的保护作用及对核因子-κB（NF-κB）表达的影响。方法采用出生3～7 d的S D大鼠视网膜组织，应用酶消化法培养Müller细胞，应用免疫细胞荧光双标方法鉴定细胞。细胞随机分为正常对照组（对照组），高糖模型组（HG组）和PE DF干预高糖组（HG＋PE DF组）。采用Western boltting和免疫细胞化学染色法检测NF-κB 的表达。结果Western boltting结果可见，与对照组相比，HG组的NF-κB 蛋白表达明显增强（P＜0．01）；与 HG组相比，HG＋PE DF组NF-κB蛋白表达显著降低（P＜0．001）。免疫细胞化学染色法结果可见，NF-κB 在对照组仅有极少量在胞浆中表达，在 HG组胞浆和胞核中皆呈强阳性表达，两者差异具有统计学意义（P＜0．001）；而 HG＋PE DF组的NF-κB表达低于其在 HG组中的表达（P＜0．01）。结论体外25 mmol·L-1高糖刺激可增加大鼠视网膜Müller细胞NF-κB的表达，从而加重Müller细胞损伤；外源给予PE DF可以显著降低NF-κB 的表达从而减少Müller细胞损伤，对视网膜Müller细胞具有显著的保护作用。“,”Objective To investigate the protective effect of pigment epithelium-derived factor(PE DF)on cultured rat Müller cell under high concentrations of glucose,and its effect on nuclear factor-kappa B (NF-κB ) expression. Methods Müller cells were cultured with retinal tissues of S Drats postnatal 3 to 7 days by enzyme digestion.The cells were identified by double-labeled immunofluorescence staining.The cells were randomly divided into normal control group (control group),high glucose model group (HG group)and PE DF-intervention high glucose group (HG +PE DF group).Western boltting method and immunocytochemical staining were used to detect the expression of NF-κB. Results Western boltting method showed the expression of NF-κB in HG group increased significantly compared with control group (P<0.01 ).NF-κB protein expression in HG +PE DF group decreased obviously compared with HG group(P<0.001).Immunocytochemical staining showed that only a few expressions of NF-κB in control group,while highly strong expression in cytoplasm and nucleus in HG group (P<0.001).Compared with HG group,NF-κB protein expressioninHG+PEDFgroupdecreasedsignificantly(P<0.01).Conclusion 25mmol·L-1highglucoseincreases the expression of NF-κB in vitro,and lead to the injury of Müller cells.Exogenous PE DF can decrease the expression of NF-κB on retinal Müller cells and decrease cells′injury significantly.So PE DF has a significant protective effect on retinal Müller cells.