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利用尼罗罗非鱼(Oreochromis niloticus)第2代遗传连锁图谱标记,对3组不同尼罗罗非鱼(♀)×萨罗罗非鱼(Sarotherodon melanotheron)(♂)杂交F1家系内亲权关系进行分析。结果显示,86个微卫星位点中共筛选出20个在尼罗罗非鱼、萨罗罗非鱼中存在差异的扩增位点,含13个种间特异性和7个共享带差异位点。尼萨杂交F1中,平均等位基因2.90,平均多态信息含量0.439,位点多态性较高。3个尼萨杂交F1家系组间遗传距离0.362~0.504,组内个体间遗传距离0.245~0.316,组内遗传距离明显小于组间。利用3个种间特异位点组合,可对3个不同家系组父、母本个体进行鉴别。通过对各组亲本与子代位点基因型分析,家系A、B和C组分别使用4、8和12个特异位点组合进行亲权鉴定,累积排除概率分别为99.99%、99.99%和99.91%,家系A、B组分别含3个半同胞家系,家系C组含2对非同胞或4个半同胞家系。
Based on the second generation genetic linkage map of Oreochromis niloticus, the relationship between F1 hybrids of three families of Nile tilapia × Sarotherodon melanotheron (♂) Analyze. The results showed that there were 20 amplified sites in 86 microsatellite loci, which differed in Nile tilapia and Salo tilapia, including 13 interspecific and 7 shared bands . Nisar F1 hybrids, the average allele 2.90, the average polymorphism information content of 0.439, locus higher polymorphism. The genetic distance between the three Nisar F1 pedigrees was 0.362-0.504, and the genetic distance among individuals within the group was 0.245-0.316. The genetic distance within the group was significantly smaller than that between the two groups. Using three combinations of interspecific loci, individuals of three different pedigree groups can be identified as individuals. By analyzing the genotypes of parents and offspring in each group, the families of A, B and C were used for paternity test with 4, 8 and 12 specific loci respectively, with the cumulative elimination probability of 99.99%, 99.99% and 99.91% , Family A, B group, respectively, with 3 half-sib families, family C group with 2 pairs of non-siblings or 4 half-sib families.