论文部分内容阅读
目的观察脑胶质瘤中胞质多聚腺苷酸化元件结合蛋白4(CPEB4)的表达情况,以及CPEB4对胶质瘤细胞U87生长的影响。方法测定脑胶质瘤和脑组织中CPEB4蛋白含量。测定细胞中CPEB4蛋白表达、细胞增殖、细胞凋亡和细胞周期及CPEB4、PIK3CA、IGF2、SMAD3、IDH1和AKT1 mRNA的表达。结果低级别脑胶质瘤组织和高级别脑胶质瘤组织中CPEB4蛋白阳性表达率高于正常脑组织(P<0.05),高级别脑胶质瘤组织中CPEB4蛋白阳性表达率高于低级别脑胶质瘤组织(P<0.05)。低级别脑胶质瘤组织和高级别脑胶质瘤组织中CPEB4蛋白水平高于正常脑组织(P<0.05),高级别脑胶质瘤组织中CPEB4蛋白水平高于低级别脑胶质瘤组织(P<0.05)。转染后24 h,CPEB4-shRNA组U87细胞中CPEB4蛋白水平低于阴性对照组和空白对照组(P<0.05)。培养第3天和第7天,CPEB4-shRNA组细胞的A490值低于阴性对照组和空白对照组(P<0.05)。CPEB4-shRNA组早期细胞凋亡率明显高于阴性对照组和空白对照组(P<0.05)。CPEB4-shRNA组G1期细胞明显高于阴性对照组和空白对照组(P<0.05),S期细胞明显低于阴性对照组和空白对照组(P<0.05)。CPEB4-shRNA组CPEB4 mRNA、PIK3CA mRNA、IGF2 mRNA和SMAD3 mRNA相对表达量均低于阴性对照组和空白对照组(P<0.05)。结论 CPEB4在脑胶质瘤中高表达,CPEB4参与脑胶质瘤细胞的生长,CPEB4对脑胶质瘤细胞的影响可能和CPEB4、PIK3CA、IGF2和SMAD3水平有一定关系。
Objective To observe the expression of cytoplasmic polyadenylation element binding protein 4 (CPEB4) in gliomas and the effect of CPEB4 on the growth of glioma U87 cells. Methods The levels of CPEB4 protein in glioma and brain tissue were determined. The expression of CPEB4 protein, cell proliferation, apoptosis and cell cycle and the expression of CPEB4, PIK3CA, IGF2, SMAD3, IDH1 and AKT1 mRNA in cells were determined. Results The positive rate of CPEB4 protein in low grade gliomas and high grade gliomas was higher than that in normal brain tissues (P <0.05). The positive rate of CPEB4 protein in high grade gliomas was higher than that in lower grade Glioma tissue (P <0.05). The levels of CPEB4 protein in low grade glioma and high grade glioma tissues were higher than those in normal brain tissues (P <0.05). The levels of CPEB4 protein in high grade glioma tissues were higher than those in lower grade glioma tissues (P <0.05). At 24 h after transfection, CPEB4 protein level in U87 cells of CPEB4-shRNA group was lower than that of negative control group and blank control group (P <0.05). On day 3 and day 7, the A490 value of CPEB4-shRNA group was lower than that of negative control group and blank control group (P <0.05). The early apoptosis rate of CPEB4-shRNA group was significantly higher than that of negative control group and blank control group (P <0.05). The number of G1 phase cells in CPEB4-shRNA group was significantly higher than that in negative control group and blank control group (P <0.05). S phase cells in CPEB4-shRNA group were significantly lower than those in negative control group and blank control group (P <0.05). The relative expression levels of CPEB4 mRNA, PIK3CA mRNA, IGF2 mRNA and SMAD3 mRNA in CPEB4-shRNA group were lower than those in negative control group and blank control group (P <0.05). Conclusions CPEB4 is highly expressed in gliomas and CPEB4 is involved in the growth of glioma cells. The effect of CPEB4 on glioma cells may be related to the levels of CPEB4, PIK3CA, IGF2 and SMAD3.