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目的研究叶酸(FA)、维生素B6(VB6)和维生素B12(VB12)对局灶性脑缺血-中脑动脉闭塞(MCAO)大鼠缺血半暗带区(ischem ic penumbra,IP)的影响。方法将SD大鼠随机分为中脑动脉闭塞模型组(MCAO)、MCAO+叶酸组(MCAO+FA)和MCAO+复合维生素(叶酸+VB6+VB12)组(MCAO+CV);预防性给药28 d后,手术制备各组大鼠大脑中动脉闭塞(MCAO)模型,造成局灶性脑缺血,对脑部进行磁共振(MR I)扫描,观察预防性给予叶酸及其联合VB6及VB12,对缺血半暗带区的改善效果。结果缺血后6 h,半暗带区表观弥散系数(ADC):MCAO+FA组为0.61±0.04,MCAO+CV组为0.63±0.04,均高于MCAO组的0.50±0.05(P<0.05),2组平均弥散系数(DCavg)依次为0.74±0.04,0.77±0.06,均高于MCAO组的0.67±0.04(P<0.05),MCAO组部分各向异性值(FA)为0.80±0.06,高于MCAO组的0.62±0.04(P<0.05)。结论补充叶酸能使脑缺血半暗带区向正常灌注区发展,抑制缺血核心区的扩大,联合补充VB6和VB12效果更佳。
Objective To investigate the effects of folic acid (FA), vitamin B6 (VB6) and vitamin B12 (VB12) on ischemic penumbra (IP) in rats with focal cerebral ischemia / reperfusion after middle cerebral artery occlusion . Methods SD rats were randomly divided into MCAO group, MCAO + FA group and MCAO + VB6 + VB12 group (MCAO + CV) for 28 days After the operation, MCAO models were made in each group, resulting in focal cerebral ischemia. MRI scan of the brain was performed. The folic acid and its combination with VB6 and VB12 were observed. The improvement of ischemic penumbra zone. Results The apparent diffusion coefficient (ADC) in penumbra zone at 6 h after ischemia was 0.61 ± 0.04 in MCAO + FA group and 0.63 ± 0.04 in MCAO + CV group, both of which were significantly higher than those in MCAO group (0.50 ± 0.05, P <0.05) ). The average DCVA of two groups were 0.74 ± 0.04 and 0.77 ± 0.06, respectively, which were higher than that of MCAO group (0.67 ± 0.04, P <0.05), and the FA of MCAO group was 0.80 ± 0.06 0.62 ± 0.04 (P <0.05) higher than the MCAO group. Conclusion Folic acid supplementation can make penumbra zone of brain ischemic area develop into normal perfusion area and inhibit the enlargement of ischemic core area. It is more effective to supplement VB6 and VB12.