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本实验表明,在二硝基氯苯致敏小鼠的同时,给予5-HT(50mg/kg),可显著地抑制接触超敏反应(CHS)。这些小鼠的外周淋巴器官中富含Ts的PNA+细胞比正常鼠明显增多。5-HT的这一生物学效应可由于低剂量的环磷酰胺预处理而消除。此外,如在DNCB致敏24小时后给与相同剂量的5-HT真该药物也不能对CHS和PNA+细胞水平产生影响。通过局部异种移植物抗宿主反应(LxGvHR)试验发现,经5-HT体内处理小鼠的脾及淋巴结细胞在豚鼠皮内诱发的LxGvHR被强烈抑制(80％)。体外实验还揭示,当正常鼠脾及淋巴结细胞与5-HT作用6小时后,其诱发LxGvHB的强度也低于正常值。结果提示,5-HT的免疫调变机理,似通过诱导Ts细胞的扩增途径对免疫反应发挥向下调节作用。
This experiment showed that 5-HT (50 mg / kg) was administered simultaneously to dinitrochlorobenzene-sensitized mice, which significantly inhibited the contact hypersensitivity (CHS). Ts-rich PNA + cells were significantly increased in peripheral lymphoid organs of these mice compared with normal mice. This biological effect of 5-HT can be eliminated by low doses of cyclophosphamide pretreatment. In addition, if the same dose of 5-HT was administered 24 hours after DNCB sensitization, the drug did not affect CHS and PNA + cell levels. The LxGvHR induced by the spleen and lymph node cells in guinea pigs was strongly suppressed (80%) by the local xenograft versus host reaction (LxGvHR) assay. In vitro experiments also revealed that the intensity of LxGvHB induced by normal spleen and lymph node cells was lower than normal after 6 hours of 5-HT treatment. The results suggest that the mechanism of 5-HT immunomodulation, like through the induction of Ts cell expansion pathway on the immune response to play a downward regulation.