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探讨郁金中姜黄素类化合物对小鼠结肠癌26-L5细胞的抗侵袭作用。 材料和方法:①药物提取和分离:郁金干燥粗末用己烷、氯仿、甲醇连续提取,体外检测3种不同溶剂的提取物对KB、P388肿瘤细胞的抗增殖作用,郁金的氯仿提取物活性最强,故采用氯仿作提取溶剂。②郁金氯仿提取物的提纯和化合物鉴定:氯仿
To investigate the anti-invasive effect of curcuminoid compounds from Yujinzhong on colon cancer 26-L5 cells. Materials and Methods: 1 Drug extraction and separation: The dried turmeric extracts were extracted with hexane, chloroform and methanol successively. The anti-proliferative effects of three different solvent extracts on KB and P388 tumor cells were tested in vitro. The extracts of turmeric were extracted with chloroform. With the strongest activity, chloroform is used as the extraction solvent. 2 Purification and identification of chloroform extract of turmeric: chloroform