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本文应用小鼠全胚胎培养技术 ,通过妊娠 d8分别 ip5,1 0 ,1 5和 2 0 mg·kg-1环磷酰胺 ( CP) ,研究了该药对器官原基形成期胚胎的致畸作用 ,并对其致畸机理作了初步探索 .给药 4h后取胚胎进行培养 ,于 d 1 0 .5收获胚胎 ,测量其卵黄囊直径 ,头长及颅臀长并记录其大体形态的变化 .结果表明 ,1 5mg· kg-1组尾畸形率最高 ;2 0 mg· kg-1组生长迟缓率最高 .电镜观察所显示的细胞凋亡的形态学变化及 DNA琼脂糖凝胶电泳的结果均提示 CP致畸作用可能与其诱导的细胞凋亡有关 .
In this study, we investigated the teratogenicity of this drug on the embryogenesis of organ primordial embryos by using whole mouse embryo culture technique at doses of 5, 10, 15 and 20 mg · kg -1 cyclophosphamide (CP) at d8 of pregnancy , And the mechanism of its teratogenicity was explored.The embryos were harvested after 4h and the embryos were harvested at d10.5, and the diameter, head length and cranial length of the yolk sac were measured and the changes of their morphology were recorded. The results showed that 1 5 mg · kg -1 group had the highest rate of tail deformity and 20 mg · kg -1 group had the highest rate of growth retardation.The morphological changes of apoptotic cells were observed by electron microscopy and the results of DNA agarose gel electrophoresis Tip CP teratogenic effect may be related to its induction of apoptosis.