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建造处理人工感染弱毒禽流感病毒的静态堆肥体系,考查禽流感病毒RNA的消失规律以及鸡尸的降解速率,评价利用堆肥技术处理染疫鸡尸的安全性和可行性。以木屑、鸡尸和牛粪建立静态堆肥体系,持续堆制40d。堆制期间检测温度。以H9亚型禽流感病毒攻毒的鸡,模拟禽流感爆发后病死或扑杀的染疫鸡尸,使用rRTPCR技术检测堆制过程中禽流感病毒RNA的降解情况。使用Real-timePCR方法扩增鸡源线粒体DNA,分析扩增片段的降解情况。堆肥40cm深度的温度在堆肥发酵1d后超过50℃,并保持了7d,最高温度57.5℃。禽流感病毒RNA第2d即可减少3log10,第12d的样本检测不到其存在。鸡源线粒体GH片段由8.97±0.15log10copies/g湿重降至5.35±0.30logcopies/g湿重,减少超过99%。表明所建堆肥体系能够有效灭活鸡粪中病原微生物,快速降解禽流感病毒RNA,迅速降解染疫鸡尸。
To construct a static composting system for handling artificial infection with attenuated avian influenza virus, to examine the disappearance of RNA of avian influenza virus and the degradation rate of chicken corpse, and to evaluate the safety and feasibility of using composting technology to treat the infected chicken. A static composting system was established with sawdust, chicken corpse and cow dung, and was kept for 40 days. Temperature during stacking inspection. Chicken challenged with H9 subtype of avian influenza virus, mock-infected chicken necrosis or culling post-avian influenza outbreak, and rRTPCR technique were used to detect the degradation of avian influenza virus RNA during the piling up process. Real-time PCR was used to amplify mitochondrial DNA from chicken and the degradation of the amplified fragment was analyzed. The depth of compost 40cm depth exceeded 50 ℃ after composting for 1 day and kept for 7 days, the highest temperature was 57.5 ℃. Bird flu RNA can be reduced by 3log10 on the 2nd day, and its presence on the 12th day can not be detected. The chicken mitochondrial GH fragment decreased from 8.97 ± 0.15log10copies / g wet weight to 5.35 ± 0.30logcopies / g wet weight, with a reduction of more than 99%. The results showed that the composting system can effectively inactivate the pathogenic microorganisms in chicken manure, rapidly degrade the RNA of bird flu virus and rapidly degrade the chicken infected.