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目的研究中和剂悬液定量鉴定试验的补充方法。方法采用TSA倾注法定量培养与营养肉汤定性培养法相结合的方法,鉴定聚六亚甲基胍盐酸盐中和剂的中和效果。结果选用0.0025 mol/L聚乙烯基硫酸钾(PVSK)作为2.0 g/L聚六亚甲基单胍盐的中和剂,TSA倾注法第2组无菌生长,而采用营养肉汤定性培养法第2组有菌生长。营养肉汤培养更适合受损细菌的恢复生长。结论在使用TSA倾注法进行中和剂悬液定量鉴定试验时,如第2组出现无菌生长,可同时对第2组进行营养肉汤定性培养,佐证中和剂的中和效果。
Objective To study the method of supplementing quantitative test of neutralizing agent suspension. Methods TSA pouring method quantitative culture and nutrient broth qualitative culture method combined to identify polyhexamethylene guanidine hydrochloride neutralization effect. Results PVSK (0.0025 mol / L) was used as the neutralizer of 2.0 g / L polyhexamethylene-guanidine salt. The second group of TSA was sterilely grown. The qualitative culture method of nutrient broth Group 2 bacteria growth. Nutrient broth cultivation is more suitable for the restoration of damaged bacteria growth. Conclusion TSA pouring method for the quantitative identification of neutralizer suspension test, such as the second group of aseptic growth, the same time, the second group of qualitative cultivation of nutrient broth to support the neutralizing agent neutralization effect.