Hydrogenotrophic denitrification is promising for tertiary nitrogen removal from municipal wastewater.To reveal the influence of residual organics in municipal wastewater on hydrogenotrophic denitrifiers,we adopted high-throughput 16S rRNA gene amplicon sequencing to examine microbial communities in hydrogenotrophic denitrification enrichments.Using effluent from a municipal wastewater treatment plant as water source,COD,nitrate and pH were controlled the same except for a gradient of biodegradable carbon (i.e.,primary effluent (PE),secondary effluent (SE),or combined primary and secondary effluent (CE)).Inorganic synthetic water (IW) was used as a control.Hydrogenophaga,a major facultative autotroph,accounted for 17.1％,5.3％,32.7％ and 12.9％ of the sequences in PE,CE,SE and IW,respectively,implicating that Hydrogenophaga grew well with or without organics.Thauera,which contains likely obligate autotrophic denitrifiers,appeared to be the most dominant genera (23.6％) in IW and accounted for 2.5％,4.6％ and 8.9％ in PE,CE and SE,respectively.Thermomonas,which is related to heterotrophic denitrification,accounted for 4.2％ and 7.9％ in PE and CE fed with a higher content of labile organics,respectively.In contrast,Thermomonas was not detected in IW and accounted for only 0.6％ in SE.Our results suggest that Thermomonas are more competitive than Thauera in hydrogenotrophic denitrification with biodegradable organics.Moreover,facultative autotrophic denitrifiers,Hydrogenophaga,are accommodating to residual organic in effluent wastewater,thus we propose that hydrogenotrophic denitrification is amenable for tertiary nitrogen removal.